PTD-DBM (Protein Transduction Domain – Dishevelled Binding Motif) is a synthetic chimeric cell-penetrating peptide with a molecular weight of 3082.64 g/mol. Its structure consists of a cell-penetrating protein transduction domain (PTD) fused to a Dishevelled (Dvl) binding motif derived from CXXC5, an intracellular negative-feedback regulator of the Wnt/β-catenin signalling pathway. The PTD component enables the peptide to traverse cell membranes without requiring receptor-mediated endocytosis, while the Dvl-binding motif provides the functional interaction domain that competes with endogenous CXXC5 for binding to Dishevelled. Produced via solid-phase peptide synthesis, PTD-DBM is associated with Wnt/β-catenin pathway modulation.PTD-DBM has been investigated as a tool compound for releasing the CXXC5-mediated negative-feedback brake on Wnt/β-catenin signalling. CXXC5 acts as a negative-feedback regulator of the Wnt/β-catenin pathway by binding to the Dishevelled protein, and PTD-DBM was designed to disrupt this CXXC5–Dishevelled interaction, thereby de-repressing pathway activity. Published studies have documented that PTD-DBM competitively blocks the CXXC5–Dishevelled interaction, stabilising β-catenin and activating Wnt/β-catenin target-gene signalling, and that topical application of the peptide accelerated cutaneous wound healing [1]. Further in vivo work has documented that disrupting the CXXC5–Dishevelled interaction with the competitor peptide activated the Wnt/β-catenin pathway and accelerated hair regrowth and wound-induced hair follicle neogenesis in mouse models, with co-administration of the GSK-3β inhibitor valproic acid further increasing the hair-regrowth response [2], making PTD-DBM a reference compound in preclinical research examining CXXC5–Dishevelled-directed activation of Wnt/β-catenin signalling in skin wound-healing and hair-follicle regeneration models.PTD-DBM is manufactured in a cGMP compliant, ISO9001 certified laboratory to a guaranteed purity of 98% or above. Every batch is independently tested using HPLC and MS-UPLC analysis before dispatch. Vials are vacuum sealed and stored in a temperature controlled, monitored cold storage system. Certificates of Analysis are available on request.Sold strictly for in vitro research purposes only. Not for human consumption. Intended for use by qualified researchers in laboratory settings only.

Editorial Team

Dr. Martina Rossi, PhD

Scientific Contributor and Reviewer

Reviewed and approved 14 June 2026

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