Buy TB-500 Research Peptide

TB-500 (CAS 885340-08-9), the synthetic form of Thymosin Beta-4 (Tβ4), is a 43-amino acid peptide with the molecular formula C₂₁₂H₃₅₀N₅₆O₇₈S and a molecular weight of 4963.44 g/mol. Its full sequence (Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser) features an N-terminal acetylation and contains the critical LKKTETQ actin-binding motif at positions 17-23. TB-500 belongs to a highly conserved family of 16 beta-thymosin isoforms and is one of the most abundant intracellular peptides in mammalian cells. Produced via solid-phase peptide synthesis, TB-500 functions as a G-actin sequestering protein.

TB-500 is central to preclinical cytoskeletal biology and tissue remodelling research. Its primary mechanism centres on the sequestration of monomeric globular actin (G-actin), regulating the equilibrium between G-actin monomers and polymerised filamentous actin (F-actin), a balance governing cell migration, morphogenesis, and cytoskeletal reorganisation. The conserved central LKKTET motif (positions 17-22) is essential for actin binding, but mutational mapping has shown that the isolated motif alone binds G-actin only weakly; high-affinity sequestration depends on additional contacts contributed by regions flanking the motif across the full-length peptide [1]. In vitro studies have examined TB-500 in endothelial cell, fibroblast, and keratinocyte preparations, documenting enhanced endothelial cell differentiation and capillary-like tube formation on reconstituted basement membrane [2], alongside directional cell migration and extracellular matrix (ECM) remodelling through matrix metalloproteinase (MMP) expression. In vivo rodent studies have investigated TB-500 in dermal wound, corneal injury, and cardiac ischaemia models, reporting accelerated full-thickness dermal wound closure accompanied by increased angiogenesis and collagen deposition [3], findings that span the neovascularisation, collagen-deposition, and inflammatory-to-proliferative phases of the tissue remodelling cascade.

TB-500 is manufactured in an ISO9001 certified laboratory to a guaranteed purity of ≥98%. Every batch is independently tested using HPLC and MS-UPLC analysis before dispatch. Vials are vacuum sealed and stored in a temperature controlled, monitored cold storage system to preserve compound integrity throughout the supply chain. Certificates of Analysis are available on request.

Sold strictly for in vitro research purposes only. Not for human consumption. Intended for use by qualified researchers in laboratory settings only.

Scientific Review

Dr. Martina Rossi, PhD

Scientific Contributor and Reviewer

Reviewed for scientific accuracy, 14 June 2026

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