Regeneration Research

Thymosin Beta-4 Research Peptide

Thymosin Beta-4 is a chain of 43 amino acids.

$52.00

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1vial

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Research Use Only · Not for human or veterinary consumption.

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Thymosin Beta-4 research peptide, >99% purity by HPLC, CAS 77591-33-4, also known as Tβ4, TB4, lyophilized powder for in-vitro laboratory research, Pure Peptides
>99% purityHPLC + MS-UPLC

Certificates of Analysis

Every batch is tested by an independent laboratory before release.

Verify Batch. Lot-level COA, Batch PP/TB4/062026. Check the batch number printed on your vial against the certificate below, which reports the tests run on it.

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What is Thymosin Beta-4?

Thymosin Beta-4 is a chain of 43 amino acids. It is found in almost every cell in the body and was first described in thymus tissue. The material sold here is made in the laboratory. It carries a small chemical cap at one end, matching the natural form. A seven-amino-acid stretch inside it, LKKTETQ, is sold separately as TB-500. TB-500 is that short piece. This is the whole peptide. Research on it has centered on the scaffolding inside cells, on the heart after injury, and on the surface of the eye and skin.

Product information reviewed by Dr. Martina Rossi, PhD

Scientific Contributor and Reviewer · Reviewed

Thymosin Beta-4 Research & Reference

What Thymosin Beta-4 does in published studies, and how to work with it in the laboratory, are covered in full on two dedicated pages rather than summarised here, so neither the evidence nor the handling detail is cut short.

Technical Specs

CAS Number77591-33-4
Also Known AsTβ4, TB4
Molecular FormulaC212H350N56O78S
Molecular Weight4963.44 g/mol
SequenceAc-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES
Purity>99%
Physical FormLyophilized Powder
TestingHPLC + MS-UPLC
SKUTB4
Batch No.PP/TB4/062026

Labeled quantity is net peptide content, not gross salt weight.

Thymosin Beta-4 Batch Results

Batch PP/TB4/062026

Laboratory
Kovera Labs
Certified
August 13, 2026
Sample
10 mg vial
Report
KVR-2026-A58019

An independent laboratory, Kovera Labs, tested a sample from this batch. These are the results on its certificate.

Purity

Pass

99.584%

RP-HPLC, C18 column, 214 nm

Identity

Pass

Confirmed

LC-MS

Purity is an HPLC figure: the main peak’s share of everything the detector recorded at one wavelength. It’s not a weight. What a purity figure counts →

Check it with the lab. Kovera Labs’ verification page looks a certificate up by its report number. Product, batch, date and results should match this page.

Chromatogram

RP-HPLC · C18 · DAD 214 nm

Chromatogram from certificate KVR-2026-A58019 for Thymosin Beta-4 batch PP/TB4/062026.
The detector trace printed on the certificate, from the run behind the purity figure. How to read this certificate →

Frequently Asked Questions

Is Thymosin Beta-4 third-party tested for purity?+

Yes. Every batch of Thymosin Beta-4 is tested by an independent third-party laboratory using HPLC and MS-UPLC, confirming purity and mass identity before the batch is released. Pure Peptides does not test its own material.

Can I see the Certificate of Analysis before buying?+

Yes. The Certificate of Analysis for the batch currently in stock can be viewed and downloaded directly on this page, before you place an order. Each certificate states the batch number, the measured purity, and the date of analysis, and can be verified independently with the issuing laboratory using the report number it carries.

What does "Research Use Only" mean?+

It means Thymosin Beta-4 is supplied for in-vitro laboratory research and for no other purpose, and must not be administered to humans or animals. Thymosin Beta-4 is legal to purchase in the United States for in vitro and non-clinical laboratory research. It has no FDA approval and no approved human use. Thymosin Beta-4 is not a scheduled substance under the Controlled Substances Act. Sold strictly for qualified research use, not human or veterinary administration.

How is Thymosin Beta-4 shipped and stored?+

Thymosin Beta-4 ships from US stock with cold-chain packaging and full tracking; orders placed before noon EST on business days are typically dispatched the same business day. On arrival, store the lyophilized powder at -20°C or below, dry and protected from light.

How is Thymosin Beta-4 related to TB-500?+

TB-500 is a seven-residue piece of this peptide. The sequence LKKTETQ sits inside Thymosin Beta-4 at positions 17 to 23, and TB-500 is that stretch on its own. This product is the whole 43-residue peptide. Research on one is not research on the other.

What has been published on Thymosin Beta-4 in people?+

Two phase 1 studies in healthy volunteers reported acceptable tolerability without measuring benefit. A randomized trial in 96 people after a heart attack reported a significant reduction in infarcted area in the subgroup treated earliest, and no significant difference across the whole trial. Two phase 2 studies followed, one in nine people with severe dry eye and one in 73 with venous leg ulcers. The venous ulcer trial reported safety comparable to placebo and a hedged efficacy conclusion, that one of the tested concentrations may have potential to accelerate healing. The Thymosin Beta-4 research article cites all of them.

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From the blog

Background and context on this compound.

Dr. Martina Rossi, PhD

Reviewed for scientific accuracy

Dr. Martina Rossi, PhD

PhD, Université Grenoble Alpes · Scientific Contributor and Reviewer

Dr. Rossi's doctoral research at Université Grenoble Alpes examined the vascular functions of bone morphogenetic proteins. Her peer-reviewed work spans cell and molecular biology, vascular biology, and gene therapy, in journals including Cardiovascular Research, Scientific Reports, and Brain Sciences. She reviews Pure Peptides' product, reference, and research pages for scientific accuracy against the sources they cite.

Reviewed ORCIDGoogle ScholarTeam profile →

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